A patch type non-enzymatic biosensor based on 3D SUS micro-needle electrode array for minimally invasive continuous glucose monitoring
We developed a new patch-shaped enzyme free biosensor using a micro-needle array with Pt black sensing electrode layer for painless continuous glucose monitoring applications. We fabricated the micro-needle array using a bulk micromachining technique and commercial stainless steel 316L grade substrate. Pt black and Ag/AgCl were then electroplated on the tip of each micro-needle as working and counter/reference electrodes of the sensor, respectively. The fabricated micro-needle was 650 μm high and 150 μm wide. We used 48 and 24 micro-needles for the working electrode and counter/reference electrode, respectively. The measured sensitivity was 1.62 μA mM−1 with high linearity of 0.9939, and was obtained within 13 sec, in glucose concentrations ranging up to 36 mM. The biosensor also exhibited a low detection limit of 50 μM. We tested the sensor under PBS solution over a period of 6 days. Then we partially inserted the sensor into a rabbit, to monitor the interstitial glucose level. We then induced change of interstitial glucose concentration by oral glucose tolerance test. Although we expected the sensor to perform for more than 6 days, it performed for just 4 days as a sensor, due to bio fouling.
Similar Publications:
The phenothiazine derivative (M2) was utilized as a redox mediator for the redox processes of the nicotinamide adenine dinucleotide (NAD+) cofacto...
In this study, we newly developed a wearable electrochemical impedimetric immunosensor with a microfluidic channel and chamber based on a Ti3C
Porous laser-induced graphene (LIG) is an attractive and promising carbon material for electrochemical applications because it c...
In this study, a hydrothermally treated chemically modified graphene (CG) and cationic polyelectr...
We proposed and fabricated a miniaturized, flexible, and fully integrated electrochemical sensor incorporated with micro-patte...
We are introducing a macroporous Au–Pt hybrid 3D electrode to be used for enzyme-free glucose detection. The proposed hybrid electrode was fabricated w...