A Polyallylamine Anchored Amine-Rich Laser-Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection
Current immunosensors have an insufficient number of binding sites for the recognition of biomolecules, which leads to false positive or negative results. In this research, a facile, cost-effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser-ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobi-lized antibodies by providing -NH2 functional groups. The proper grafting of PAAMI is confirmed through X-ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immuno-globulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point-of-care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer bio-markers after immobilizing the relevant antibodies.
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