Publication

Most of the known enzyme-based glucose biosensors are based on the glucose oxidase (GOx), which can selectively catalyze the oxidation of glucose to gluconolactone in the presence of oxygen and produce H2O2 simultaneously [1]. However, the oxidation of H2O2 usually requires a relatively high positive potential [1]. In addition, GOx-based glucose sensors are susceptible to the oxygen concentration in the measuring media [2]. In this study, to overcome these problems, the polymer microneedle-based flexible glucose sensor for continuous glucose monitoring was newly developed using glucose dehydrogenase (GDH) that is not affected by oxygen concentration and can be measured selectively. The fabricated sensor showed excellent amperometric response of the glucose concentration of 0 mM to 30 mM linear regression curve, where R2 was calculated to 0.980 and 0.971 on two ranges, respectively. The limit of detection (signal-to-noise ratio = 3) was calculated to 90 μM. It is also remarkably selective opposite to the interferences such as uric acid, acetaminophen, and ascorbic acid.