A Prostate Cancer Detection Immunosensor Based on Nafion/Reduced Graphene Oxide/Aldehyde Functionalized Methyl Pyridine Composite Electrode
In this research, an electrochemical PSA (prostate specific antigen) immunosensor based on Nafion/reduced graphene oxide (rGO)/aldehyde methyl pyridine (CHO-MP) composite electrode was newly developed by using noncovalent functionalization of rGO and CHO-MP. Due to improper binding stability of the immobilized antibody, immunosensors may show false positive results. The proposed composite material can provide suitable microenvironment for the immobilized anti-PSA and improve the conductivity of electrode. Furthermore, the used nafion can improve the mechanical and chemical behaviors of the developed immunosensor. Physical characterization of rGO/CHO-MP was observed through XPS, FTIR, and FE-SEM. Under the optimized conditions, the fabricated immunosensor showed a linear range of 0.005- 90 ng mL−1, detection limit of 1.6 pg mL−1, excellent selectivity, stability, and reproducibility. Lastly, human serum samples were analyzed and the recovery range was obtained as 95.8% to 107.9% with an RSD of 5.1% (n = 5). These results showed that the as-fabricated sensor is highly promising in clinical applications.
Similar Publications:
In this context, a novel, scalable and facile method for the green synthesis of graphene oxide (GO) to facilitate effective and ...
In this study, an environmentally friendly and simple method to synthesize rGO–BiNP nanocomposite was newly developed to a...
A novel nanocomposite of hexagonal cobalt oxide nanosheets (HCONS) homogeneously dispersed on a carbon matrix of reduced graphen...
In this study, we successfully developed a novel nanocomposite comprising hexagonal nickel oxide ...
The conventional synthetic methods for graphene oxide are based on the reaction of graphite with ...
A solvothermal technique with extra treatment was performed to synthesize high quality chemically modified graphene (CG). An environmentally friendly reducin...
An ultra-sensitive electrochemical immunosensor was successfully developed to accurately detect prostate specific antigen. To improve the performance of immu...
In this paper, the fabrication and characterization of chemically modified reduced graphene oxide (CRGO) embedded with platinum and palladium nanoparticles (...