An Electrochemical Enzymatic Biosensor Based on AuFGssol-gel-GOx Composite Nafion

We have reported an electrochemical glucose biosensor based on the sol-gel derived composites with glucose oxidase (GOx) and nafion on chemically functionalized graphene sheets (FGs). FGs was developed by chemically treatment of exfoliate graphite oxide and heat treatment of FGs film on the substrate electrode. The as-prepared FGs modified gold thin film electrode exhibited good electrocatalytic behaviors toward oxidation of hydrogen peroxide. Furthermore, the immobilization of GOx was conducted by the entrapment of glucose oxidase in silica-gel network, which became polymerized in the presence of enzyme and nafion is coated on the modified surface. The as-prepared glucose sensor exhibited good performance towards the oxidation of glucose, along with low applied potential, long linear detection range, low detection limit and long period stability, which were assigned to the high active surface area of FGs and allow the entrapment of GOx.

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