Gold Nanoparticles Assembled Chemically Functionalized Reduced Graphene Oxide Supported Electrochemical Immunosensor for Ultra-Sensitive Prostate Cancer Detection
An ultra-sensitive electrochemical immunosensor was successfully developed to accurately detect prostate specific antigen. To improve the performance of immunosensor, it was fabricated by using hybrid nano-structured electrodes of high performance chemically functionalized reduced graphene oxide (CRGO) and gold nanoparticles (AuNPs) followed by covalently immobilized monoclonal anti-PSA antibody. We optimized the immunosensor, under different conditions using cyclic voltammetry and differential pulse voltammetry. We obtained a low detection limit of 3 pg mL−1 and wide linear range from 6 pg mL−1 to 30 ng mL−1, good reproducibility, selectivity, and stability. Finally, human serum samples were analyzed. The obtained recovery range was from 97% to 110% with relative standard deviation (RSD) of 3.88%. Thus, the developed immunosensor can provide a great potential in clinical and diagnostic applications.
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