Publication

Continuous real-time lactate monitoring in interstitial fluid (ISF) is very important for athletes and patients. Lactate sensor can improve athlete’s training programs and prevent potential diseases of patients in advance. However, the recently reported lactate sensors cannot be used to monitor high concentrations of lactate in sweat due to its low detection range so that it is difficult to prevent serious disease. Herein, a high-performance microneedle-based lactate senor was developed by using reduced graphene oxide (rGO) and platinum nanoparticles (PtNPs) deposited on gold electrode to improve catalytic activity and by optimizing nafion membrane packaging to improve the detection range as a permselective membrane. Furthermore, chitosan and N-(3-dimethylaminpropyl)-N’-ethylcarbodiimide hydrochloride (EDC) coupled with N-hydroxysuccinimide (NHS) result in stable binding between substrate and enzyme. The fabricated sensor exhibited a wide linear range (10 mM<), high sensitivity (43.96 μAmM-1cm-2), and 2.04 μM limit of detection.